About: Chromatin Regulation of Phaseolin Transcrption     Goto   Sponge   NotDistinct   Permalink

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  • Phaseolin, a major seed storage protein of Phaseolus vulgaris is encoded by phaseolin (phas) gene. During seed development, phas is expressed at exceptionally high levels but it is fully repressed in all vegetative tissues. The establishment of a repressive chromatin structure and the presence of a nucleosome over three phased TATA boxes of the phas promoter is responsible for the lack of phas expression in vegetative tissue. Tranion from the phas promoter is enabled during early embryogenesis by a two-step process. Step 1 involves chromatin remodeling (by the B3 domain-containing tranion factor PvALF), that permits formation of a potentiated tranion complex. In step 2, tranional activation is initiated by an ABAinduced signal transduction cascade. The main goal of this proposal is to contribute to the explanation how changes in chromatin structure over the phas promoter are orchestrated to permit tranion. This will include search for proteins that interact with the effector to achieve chrom (en)
  • Nalezení pozitivních i negativních upstream faktorů vedoucích k aktivaci transkripce promoteru phas.
Title
  • Chromatin Regulation of Phaseolin Transcrption (en)
  • Regulace transkripce faseolinu na úrovni chromatinu
skos:notation
  • ME10038
http://linked.open...avai/cep/aktivita
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http://linked.open...ep/celkoveNaklady
http://linked.open...datumDodatniDoRIV
http://linked.open...i/cep/druhSouteze
http://linked.open...ep/duvernostUdaju
http://linked.open.../cep/fazeProjektu
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http://linked.open.../cep/klicovaSlova
  • phaseolin; chromatin; histon code; expression regulation; embryogenesis (en)
http://linked.open...ep/partnetrHlavni
http://linked.open...inujicichPrijemcu
http://linked.open...cep/pocetPrijemcu
http://linked.open...ocetSpoluPrijemcu
http://linked.open.../pocetVysledkuRIV
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http://linked.open...ep/ukonceniReseni
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  • Our work focused on discovering genes underlying PvALF mediated expression of the Phas promoter. Chromatin modifying enzymes, modules/clusters of genes that assist with understanding of gene-gene, gene-function interactions for chromatin remodeling during seed development were identified by next generation sequencing. This gene network is transcriptionally controlled by PvALF during embryogenesis. (en)
  • Práce byla především zaměřena na nalezení genů, které podmiňují expresi faseolinu řízenou genem PvALF. Pomocí sekvenování nové generace byly identifikovány a charakterizovány enzymy modifikující chromatin, jakož i moduly/klastry genů, které následně vedly k pochopení vztahů mezi geny a interakcí gen-funkce během remodelace. Tento genový network je během embryogeneze rovněž řízen PvALF. (cs)
http://linked.open...tniCyklusProjektu
http://linked.open.../cep/klicoveSlovo
  • chromatin
  • expression regulation
  • histon code
  • phaseolin
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