About: Polymerase Chain Reaction

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An Entity of Type : owl:Class, within Data Space : linked.opendata.cz:8890 associated with source document(s)

Attributes	Values
rdf:type	Class
rdfs:label	Polymerase Chain Reaction
rdfs:subClassOf	Molecular Genetic Technique Molecular Analysis
Concept_In_Subset	Clinical Data Interchange Standards Consortium Terminology CDISC SDTM Terminology CDISC SDTM Method Terminology
Semantic_Type	Molecular Biology Research Technique
Preferred_Name	Polymerase Chain Reaction
UMLS_CUI	C0032520
Contributing_Source	CDISC
ALT_DEFINITION	A method for amplifying a DNA base sequence using multiple rounds of heat denaturation of the DNA and annealing of oligonucleotide primers complementary to flanking regions in the presence of a heat-stable polymerase. This results in duplication of the targeted DNA region. Newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, so that successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.CDISC A laboratory method used to make many copies of a specific DNA sequence.NCI-GLOSS
Legacy_Concept_Name	Polymerase_Chain_Reaction
FULL_SYN	PCRPTNCI-GLOSSCDR0000403140 Polymerase Chain ReactionSYNCITCGA polymerase chain reactionPTNCI-GLOSSCDR0000044798 Polymerase Chain ReactionPTNCI PCRABNCI POLYMERASE CHAIN REACTIONPTCDISC PCRSYCDISC
DEFINITION	A method for amplifying a DNA base sequence using multiple rounds of heat denaturation of the DNA and annealing of oligonucleotide primers complementary to flanking regions in the presence of a heat-stable polymerase. This results in duplication of the targeted DNA region. Newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, so that successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.NCI
code	C17003
http://linked.open...y/mesh/hasConcept	http://linked.opendata.cz/resource/mesh/concept/M0024634
is rdfs:subClassOf of	Reverse Transcriptase-Polymerase Chain Reaction Whole Genome Amplification Mycobacterial Interspersed Repetitive Unit Typing Spacer Oligonucleotide Typing Real Time PCR CDR3 Spectratyping Hepatitis A Viral Load PCR Measurement Hepatitis B Viral Load PCR Measurement Hepatitis C Viral Load PCR Measurement HIV Viral Load PCR Measurement COLD-PCR

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