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An Entity of Type : owl:Class, within Data Space : linked.opendata.cz:8890 associated with source document(s)

AttributesValues
rdf:type
rdfs:label
  • COLD-PCR
rdfs:subClassOf
Semantic_Type
  • Molecular Biology Research Technique
Preferred_Name
  • COLD-PCR
NCI_META_CUI
  • CL433224
FULL_SYN
  • Co-amplification at Lower Denaturation Temperature-PCRSYNCI
  • COLD-PCRPTNCI
DEFINITION
  • A modification of the standard polymerase chain reaction that increases the ability to preferentially amplify minority alleles and somatic mutations in the presence of an excess of wild-type molecules. Sequence-variant molecules are preferentially selected by the lower melting temperature of their mismatched heteroduplex after they've annealed to a wild-type molecule that is present in excess. By carefully selecting the denaturing temperature below the Tm for the wild-type molecule, only the mismatch heteroduplexes will melt and act as efficient templates for strand synthesis.NCI
code
  • C99486
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