About: Electrophoretic Mobility Shift Assay     Goto   Sponge   Distinct   Permalink

An Entity of Type : owl:Class, within Data Space : linked.opendata.cz:8890 associated with source document(s)

AttributesValues
rdf:type
rdfs:label
  • Electrophoretic Mobility Shift Assay
rdfs:subClassOf
Semantic_Type
  • Laboratory Procedure
Preferred_Name
  • Electrophoretic Mobility Shift Assay
UMLS_CUI
  • C0949632
Legacy_Concept_Name
  • Electrophoretic_Mobility_Shift_Assay
FULL_SYN
  • Gel Shift AssaySYNCI
  • Electrophoretic Mobility Shift AssayPTNCI
  • EMSAABNCI
DEFINITION
  • A molecular biology technique used to detect the interaction of a DNA binding protein with its cognate binding sequence. Labeled DNAs were reacted with crude cell extracts and the complexes are run through a non-denaturing polyacrylamide gel. The migration of the labeled DNA through the gel will be slower by being bound. This shift in electrophoretic mobility indicates functional binding between the protein and the DNA.NCI
code
  • C19362
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