About: Polyacrylamide Gel Electrophoresis     Goto   Sponge   Distinct   Permalink

An Entity of Type : owl:Class, within Data Space : linked.opendata.cz:8890 associated with source document(s)

AttributesValues
rdf:type
rdfs:label
  • Polyacrylamide Gel Electrophoresis
rdfs:subClassOf
Semantic_Type
  • Molecular Biology Research Technique
Preferred_Name
  • Polyacrylamide Gel Electrophoresis
UMLS_CUI
  • C0013862
ALT_DEFINITION
  • Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.MSH2001
Legacy_Concept_Name
  • Polyacrylamide_Gel_Electrophoresis
FULL_SYN
  • Fractionation, Polyacrylamide GelSYNCI
  • Polyacrylamide Gel ElectrophoresisPTNCI
  • Electrophoresis, Polyacrylamide GelSYNCI
  • PAGEABNCI
DEFINITION
  • Analytical and separative procedures in which molecules, particularly proteins or nucleic acids, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. For proteins, commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight.NCI
code
  • C16539
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